Biometric Technology, Inc.

Systematic comparability and prediction of the consequences of missense mutations on protein-DNA and protein-RNA interactions

 The binding affinities of protein-nucleic acid interactions may very well be altered as a consequence of missense mutations occurring in DNA- or RNA-binding proteins, subsequently leading to numerous illnesses. Sadly, a scientific comparability and prediction of the consequences of mutations on protein-DNA and protein-RNA interactions (these two mutation lessons are termed MPDs and MPRs, respectively) remains to be missing. Right here, we demonstrated that these two lessons of mutations may generate comparable or completely different tendencies for binding free power adjustments when it comes to the properties of mutated residues. We then developed regression algorithms individually for MPDs and MPRs by introducing novel geometric partition-based power options and interface-based structural options.

By characteristic choice and ensemble studying, comparable computational frameworks that built-in energy- and nonenergy-based fashions had been established to estimate the binding affinity adjustments ensuing from MPDs and MPRs, however the chosen options for the remaining fashions had been completely different and subsequently mirrored the specificity of those two mutation lessons. Moreover, the proposed methodology was prolonged to the identification of mutations that considerably decreased the binding affinities. In depth validations indicated that our algorithm typically carried out higher than the state-of-the-art strategies on each the regression and classification duties. The webserver and software program are freely obtainable at PEMPNI

Optimising glyphosate tolerance in rapeseed (Brassica napus L.) by CRISPR/Cas9-based geminiviral donor DNA replicon system with Csy4-based single-guide RNA processing

 

Rapeseed (Brassica napus L.) is a crucial oil crop worldwide, and efficient weed management can defend its yields and high quality. Farmers can profit from tolerant cultivars when utilizing herbicides, equivalent to glyphosate. Amino acid substitutions in enolpyruvylshikimate-3-phosphate synthase (EPSPS) render the plant much less delicate to glyphosate. Due to this fact, we aimed to optimise the glyphosate tolerance trait in rapeseed by way of endogenous EPSPS modification. To attain efficient gene substitute in B. napus L., we employed a CRISPR/Cas9 system expressing single-guide RNAs (sgRNAs) cleaved by the CRISPR-associated RNA endoribonuclease Csy4 from Pseudomonas aeruginosa for focused induction of double-strand breaks. Each the donor template and a geminiviral replicon harbouring an sgRNA expression cassette had been launched into plant cells.

Utilizing sgRNAs focusing on adjoining donor DNA template containing synonymous mutations in sgRNA websites, we achieved exact gene replacements within the endogenous B. napus EPSPS gene, BnaC04EPSPS, leading to TIPS and LFGAAGMCRL amino acid substitutions at frequencies as much as 20%. Rapeseed seedlings harbouring EPSPSmTIPS or EPSPSmLFGAAGMCRL had been glyphosate tolerant. Moreover, modifications in BnaC04EPSPS had been exactly transmitted to the following era. Our genome enhancing technique allows extremely environment friendly gene focusing on and the induction of glyphosate tolerance in oilseed rape.

DNA / RNA Damage Antibody
abx440040-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx440321-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx440602-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx440883-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx441164-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx441445-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx441726-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx442007-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx444253-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx444534-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
20-abx444884	Abbexa	EUR 594.00 EUR 393.60	100 ug 25 ug

DNA / RNA Damage Antibody
abx442288-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx442569-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx442850-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx443130-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx443410-100ug	Abbexa	100 ug	EUR 644.4

DNA / RNA Damage Antibody
abx443691-100ug	Abbexa	100 ug	EUR 678

DNA / RNA Damage Antibody
abx443972-100ug	Abbexa	100 ug	EUR 661.2

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
20-abx320325	Abbexa	EUR 360.00 EUR 292.80	100 ul 50 ul

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
20-abx321453	Abbexa	EUR 360.00 EUR 292.80	100 ul 50 ul

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
20-abx005629	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
abx148136-100ug	Abbexa	100 ug	EUR 526.8

Monoclonal antibody for DNA/RNA Damage
SMC-155C	Stressmarq	0.025mg	EUR 228
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

Monoclonal antibody for DNA/RNA Damage
SMC-155D	Stressmarq	0.1mg	EUR 398.4
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A390	Stressmarq	0.1mg	EUR 454.8
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 390.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A488	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 488.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A565	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 565.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A594	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 594.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A633	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 633.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A655	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 655.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A680	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 680.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A700	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 700.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-ALP	Stressmarq	0.1mg	EUR 446.4
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Alkaline Phosphatase.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-APC	Stressmarq	0.1mg	EUR 452.4
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-APCCY7	Stressmarq	0.1mg	EUR 540
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC/Cy7.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-BI	Stressmarq	0.1mg	EUR 448.8
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Biotin.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY350	Stressmarq	0.1mg	EUR 471.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 350.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY405	Stressmarq	0.1mg	EUR 457.2
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 405.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY488	Stressmarq	0.1mg	EUR 445.2
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 488.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY594	Stressmarq	0.1mg	EUR 447.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 594.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY633	Stressmarq	0.1mg	EUR 441.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 633.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-FITC	Stressmarq	0.1mg	EUR 444
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with FITC.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-HRP	Stressmarq	0.1mg	EUR 439.2
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with HRP.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-P594	Stressmarq	0.1mg	EUR 462
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with PE/ATTO 594.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-PCP	Stressmarq	0.1mg	EUR 452.4
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with PerCP.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-RPE	Stressmarq	0.1mg	EUR 450
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with RPE.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-STR	Stressmarq	0.1mg	EUR 451.2
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Streptavidin.

Monoclonal antibody for DNA/RNA Damage
SMC-155S	Stressmarq	0.012mg	EUR 78
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

Oxidative-Stress Responsive 1 (OXSR1) Antibody
20-abx114290	Abbexa	EUR 878.40 EUR 477.60	150 ul 50 ul

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody
abx236027-100ug	Abbexa	100 ug	EUR 610.8

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody
20-abx114289	Abbexa	EUR 878.40 EUR 477.60	150 ul 50 ul

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody
abx146030-100ug	Abbexa	100 ug	EUR 469.2

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody
20-abx173928	Abbexa	EUR 1028.40 EUR 526.80	1 mg 200 ug

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody
20-abx128290	Abbexa	EUR 510.00 EUR 159.60 EUR 1446.00 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody
abx029975-400ul	Abbexa	400 ul	EUR 627.6

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody
abx029975-80l	Abbexa	80 µl	EUR 343.2

Thiol Oxidative Stress Assay
1021	Ethos Biosciences	1 kit	EUR 460.8

Human Oxidative Stress-Responsive 1 Protein (OXSR1) Antibody
33219-05111	AssayPro	150 ug	EUR 313.2

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody
abx122927-100ug	Abbexa	100 ug	EUR 469.2

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody
20-abx302885	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Recombinant Human DNA oxidative demethylase ALKBH2 Protein, GST, E.coli-10ug
QP7771-ec-10ug	EnQuireBio	10ug	EUR 240

Recombinant Human DNA oxidative demethylase ALKBH2 Protein, GST, E.coli-1mg
QP7771-ec-1mg	EnQuireBio	1mg	EUR 1958.4

Recombinant Human DNA oxidative demethylase ALKBH2 Protein, GST, E.coli-50ug
QP7771-ec-50ug	EnQuireBio	50ug	EUR 315.6

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody (HRP)
20-abx312498	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody (FITC)
20-abx312499	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Recombinant Human DNA oxidative demethylase ALKBH2 Protein, GST, E.coli-100ug
QP7771-ec-100ug	EnQuireBio	100ug	EUR 489.6

Recombinant Human DNA oxidative demethylase ALKBH2 Protein, GST, E.coli-200ug
QP7771-ec-200ug	EnQuireBio	200ug	EUR 760.8

Recombinant Human DNA oxidative demethylase ALKBH2 Protein, GST, E.coli-500ug
QP7771-ec-500ug	EnQuireBio	500ug	EUR 1272

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody (Biotin)
20-abx312500	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Oxidative Stress-Responsive Serine-Rich Protein 1 (OSER1) Antibody
20-abx318659	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Rat Oxidative Stress Primer Library
ROSL-I	Real Time Primers	1 set	EUR 657.6

Mouse Oxidative Stress Primer Library
MOSL-I	Real Time Primers	1 set	EUR 657.6

Human Oxidative Stress Primer Library
HOSL-I	Real Time Primers	1 set	EUR 540

Human Oxidative Stress-Responsive 1 Protein (OXSR1) Antibody (Biotin Conjugate)
33219-05121	AssayPro	150 ug	EUR 442.8

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Polyclonal Antibody (Human)
4-PAG202Hu01	Cloud-Clone	EUR 296.40 EUR 3012.00 EUR 750.00 EUR 372.00 EUR 256.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1)

TBARS/Malondialdehyde Oxidative Stress Assay
1020	Ethos Biosciences	1 kit	EUR 373.2

8-isoprostane oxidative stress ELISA Kit
8iso1	Detroit R&D	1 Kit	EUR 368.4

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Polyclonal Antibody (Human), APC
4-PAG202Hu01-APC	Cloud-Clone	EUR 414.00 EUR 3930.00 EUR 1094.40 EUR 528.00 EUR 262.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1). This antibody is labeled with APC.

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Polyclonal Antibody (Human), Cy3
4-PAG202Hu01-Cy3	Cloud-Clone	EUR 502.80 EUR 5190.00 EUR 1410.00 EUR 654.00 EUR 301.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1). This antibody is labeled with Cy3.

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Polyclonal Antibody (Human), HRP
4-PAG202Hu01-HRP	Cloud-Clone	EUR 379.20 EUR 3426.00 EUR 968.40 EUR 477.60 EUR 247.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1). This antibody is labeled with HRP.

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Polyclonal Antibody (Human), PE
4-PAG202Hu01-PE	Cloud-Clone	EUR 355.20 EUR 3168.00 EUR 900.00 EUR 446.40 EUR 234.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1). This antibody is labeled with PE.

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Polyclonal Antibody (Human), FITC
4-PAG202Hu01-FITC	Cloud-Clone	EUR 355.20 EUR 3168.00 EUR 900.00 EUR 446.40 EUR 234.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1). This antibody is labeled with FITC.

Human Oxidative Stress-Responsive 1 Protein (OXSR1) AssayLite Antibody (RPE Conjugate)
33219-05151	AssayPro	150 ug	EUR 513.6

Human Oxidative Stress-Responsive 1 Protein (OXSR1) AssayLite Antibody (APC Conjugate)
33219-05161	AssayPro	150 ug	EUR 513.6

Human Oxidative Stress-Responsive 1 Protein (OXSR1) AssayLite Antibody (FITC Conjugate)
33219-05141	AssayPro	150 ug	EUR 513.6

Human Oxidative Stress-Responsive 1 Protein (OXSR1) AssayLite Antibody (PerCP Conjugate)
33219-05171	AssayPro	150 ug	EUR 565.2

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Polyclonal Antibody (Human), APC-Cy7
4-PAG202Hu01-APC-Cy7	Cloud-Clone	EUR 685.20 EUR 7716.00 EUR 2046.00 EUR 912.00 EUR 382.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1). This antibody is labeled with APC-Cy7.

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Polyclonal Antibody (Human), Biotinylated
4-PAG202Hu01-Biotin	Cloud-Clone	EUR 373.20 EUR 2952.00 EUR 872.40 EUR 457.20 EUR 262.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1). This antibody is labeled with Biotin.

DNA Damage-Binding Protein 1 (DDB1) Antibody
abx232284-100ug	Abbexa	100 ug	EUR 577.2

DNA Damage-Binding Protein 1 (DDB1) Antibody
20-abx333999	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

DNA Damage-Binding Protein 2 (DDB2) Antibody
20-abx338922	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

OxiSelect Nitrosative DNA/RNA Damage ELISA Kit (8-Nitroguanine Quantitation)
STA-825	Cell Biolabs	96 assays	EUR 957.6
Description: The OxiSelect Nitrosative DNA/RNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-nitroguanine in urine, serum, or plasma samples.

OxiSelect Nitrosative DNA/RNA Damage ELISA Kit (8-Nitroguanine Quantitation)
STA-825-5	Cell Biolabs	5 x 96 assays	EUR 3888
Description: The OxiSelect Nitrosative DNA/RNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-nitroguanine in urine, serum, or plasma samples.

Human Oxidative Stress-Responsive 1 Protein (OXSR1) AssayLite Antibody (FITC, RPE, APC, PerCP Conjugate)
33219-05181	AssayPro	1 x 75 ug, 3 x 30 ug	EUR 702

DNA Damage Inducible Transcript 4 (DDIT4) Antibody
20-abx213754	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx172142	Abbexa	EUR 1028.40 EUR 526.80	1 mg 200 ug

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx326210	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx000607	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx004988	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx103576	Abbexa	EUR 510.00 EUR 159.60 EUR 1446.00 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx112119	Abbexa	EUR 878.40 EUR 477.60	150 ul 50 ul

DNA Damage Inducible Transcript 4 (DDIT4) Antibody
20-abx112120	Abbexa	EUR 878.40 EUR 477.60	150 ul 50 ul

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx176186	Abbexa	EUR 1479.60 EUR 710.40	1 mg 200 ug

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx125748	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx125750	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

DNA Damage Inducible Transcript 4 (DDIT4) Antibody
20-abx241498	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

DNA Damage Inducible Transcript 4 (DDIT4) Antibody
20-abx123209	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx129884	Abbexa	EUR 526.80 EUR 159.60 EUR 1479.60 EUR 710.40 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx301173	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
abx026524-400ul	Abbexa	400 ul	EUR 627.6

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
abx026524-80l	Abbexa	80 µl	EUR 343.2

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
abx027183-400ul	Abbexa	400 ul	EUR 627.6

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
abx027183-80l	Abbexa	80 µl	EUR 343.2

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
abx332226-100ul	Abbexa	100 ul	EUR 510

DNA Damage Inducible Transcript 3 (DDIT3) Antibody
20-abx329652	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

P53 And Dna Damage Regulated 1 (PDRG1) Antibody
20-abx114308	Abbexa	EUR 878.40 EUR 477.60	150 ul 50 ul

P53 And DNA Damage Regulated 1 (PDRG1) Antibody
20-abx328877	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

P53 And DNA Damage Regulated 1 (PDRG1) Antibody
20-abx014692	Abbexa	EUR 376.80 EUR 117.60 EUR 477.60 EUR 594.00	100 ug 10 ug 200 ug 300 µg

P53 And DNA Damage Regulated 1 (PDRG1) Antibody
20-abx008020	Abbexa	EUR 360.00 EUR 526.80 EUR 226.80	100 ul 200 ul 30 ul

P53 And DNA Damage Regulated 1 (PDRG1) Antibody
abx236289-100ug	Abbexa	100 ug	EUR 577.2

A genome-wide display screen for differentially methylated lengthy noncoding RNAs recognized that lncAC007255.Eight is regulated by promoter DNA methylation in Beas-2B cells malignantly reworked by NNK

 Tobacco publicity is well-known to induce genetic and epigenetic adjustments that contribute to the pathogenesis of lung most cancers. 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a big tobacco-specific carcinogen, however the oncogenic mechanisms of NNK haven’t been completely elucidated. On this examine we discovered that DNA methyltransferase 1(DNMT1) was overexpressed in malignantly reworked human bronchial epithelial Beas-2B cells induced by NNK (2B-NNK cells), by therapy with NNK (400 μg/ml) for 7 days. An Arraystar Human noncoding RNA Promoter Microarray was used to detect the DNA methylation standing of the promoter area of lengthy noncoding RNAs (lncRNAs). The outcome confirmed that 1010 differentially methylated fragments had been current within the lncRNA promoter area. QRT-PCR revealed that the expression of lncRNA AC007255.Eight was remarkably downregulated in 2B-NNK cells and lung most cancers tissues.

Moreover, Methylation-particular PCR confirmed that the methylation of the lncRNA AC007255.Eight promoter was elevated in 2B-NNK cells and lung most cancers tissues. The lowered expression of lncRNA AC007255.Eight was considerably related to hypermethylation of lncRNA AC007255.Eight promoter area. LncRNA AC007255.Eight overexpression may lead to decreased cell proliferation and elevated cell apoptosis in 2B-NNK cells. In conclusion, NNK induced lncRNA AC007255.8 promoter hypermethylation by way of upregulation of DNMT1 in Beas-2B cells, resulting in downregulation of lncRNA AC007255.8, and finally the enhancement of cell proliferation and the inhibition of apoptosis. This analysis affords novel insights into the epigenetic mechanisms of lung most cancers, and can stimulate additional analysis into the involvement of aberrant DNA methylation of non-coding areas of the genome within the pathogenesis of lung most cancers.

Detecting protein and DNA/RNA constructions in cryo-EM maps of intermediate decision utilizing deep studying

 

An growing variety of density maps of macromolecular constructions, together with proteins and DNA/RNA complexes, have been decided by cryo-electron microscopy (cryo-EM). Though these days maps at a near-atomic decision are routinely reported, there are nonetheless substantial fractions of maps decided at intermediate or low resolutions, the place extracting construction data shouldn’t be trivial. Right here, we report a brand new computational methodology, Emap2sec+, which identifies DNA or RNA as properly because the secondary constructions of proteins in cryo-EM maps of 5 to 10 Å decision.

Emap2sec+ employs the deep Residual convolutional neural community. Emap2sec+ assigns structural labels with related possibilities at every voxel in a cryo-EM map, which can assist construction modeling in an EM map. Emap2sec+ confirmed secure and excessive task accuracy for nucleotides in low decision maps and improved efficiency for protein secondary construction assignments than its earlier model when examined on simulated and experimental maps.

 

Temporal panorama of human intestine RNA and DNA virome in SARS-CoV-2 an infection and severity

 

Background: Coronavirus illness 2019 (COVID-19) brought on by the enveloped RNA virus SARS-CoV-2 primarily impacts the respiratory and gastrointestinal tracts. SARS-CoV-2 was remoted from fecal samples, and energetic viral replication was reported in human intestinal cells. The human intestine additionally harbors an unlimited quantity of resident viruses (collectively referred to as the virome) that play a task in regulating host immunity and illness pathophysiology. Understanding intestine virome perturbation that underlies SARS-CoV-2 an infection and severity is an unmet want.

 

Strategies: We enrolled 98 COVID-19 sufferers with various illness severity (Three asymptomatic, 53 gentle, 34 reasonable, 5 extreme, Three important) and 78 non-COVID-19 controls matched for gender and co-morbidities. All topics had fecal specimens sampled at inclusion. Blood specimens had been collected for COVID-19 sufferers at admission to check for inflammatory markers and white cell counts. Amongst COVID-19 circumstances, 37 (38%) sufferers had serial fecal samples collected 2 to three occasions per week from time of hospitalization till after discharge. Utilizing shotgun metagenomics sequencing, we sequenced and profiled the fecal RNA and DNA virome. We investigated alterations and longitudinal dynamics of the intestine virome in affiliation with illness severity and blood parameters.

Outcomes: Sufferers with COVID-19 confirmed underrepresentation of Pepper gentle mottle virus (RNA virus) and a number of bacteriophage lineages (DNA viruses) and enrichment of environment-derived eukaryotic DNA viruses in fecal samples, in comparison with non-COVID-19 topics. Such intestine virome alterations continued as much as 30 days after illness decision. Fecal virome in SARS-CoV-2 an infection harbored extra stress-, inflammation-, and virulence-associated gene encoding capacities together with these pertaining to bacteriophage integration, DNA restore, and metabolism and virulence related to their bacterial host. Baseline fecal abundance of 10 virus species (1 RNA virus, pepper chlorotic spot virus, and 9 DNA virus species) inversely correlated with illness COVID-19 severity. These viruses inversely correlated with blood ranges of pro-inflammatory proteins, white cells, and neutrophils. Among the many 10 COVID-19 severity-associated DNA virus species, Four confirmed inverse correlation with age; 5 confirmed persistent decrease abundance each throughout illness course and after illness decision relative to non-COVID-19 topics.

Conclusions: Each enteric RNA and DNA virome in COVID-19 sufferers had been completely different from non-COVID-19 topics, which continued after illness decision of COVID-19. Intestine virome might calibrate host immunity and regulate severity to SARS-CoV-2 an infection. Our statement that intestine viruses inversely correlated with each severity of COVID-19 and host age might partly clarify that older topics are susceptible to extreme and worse COVID-19 outcomes. Altogether, our information spotlight the significance of human intestine virome in severity and probably therapeutics of COVID-19. Video Summary.