Biometric Technology, Inc.

Systematic comparability and prediction of the consequences of missense mutations on protein-DNA and protein-RNA interactions

 The binding affinities of protein-nucleic acid interactions may very well be altered as a consequence of missense mutations occurring in DNA- or RNA-binding proteins, subsequently leading to numerous illnesses. Sadly, a scientific comparability and prediction of the consequences of mutations on protein-DNA and protein-RNA interactions (these two mutation lessons are termed MPDs and MPRs, respectively) remains to be missing. Right here, we demonstrated that these two lessons of mutations may generate comparable or completely different tendencies for binding free power adjustments when it comes to the properties of mutated residues. We then developed regression algorithms individually for MPDs and MPRs by introducing novel geometric partition-based power options and interface-based structural options.

By characteristic choice and ensemble studying, comparable computational frameworks that built-in energy- and nonenergy-based fashions had been established to estimate the binding affinity adjustments ensuing from MPDs and MPRs, however the chosen options for the remaining fashions had been completely different and subsequently mirrored the specificity of those two mutation lessons. Moreover, the proposed methodology was prolonged to the identification of mutations that considerably decreased the binding affinities. In depth validations indicated that our algorithm typically carried out higher than the state-of-the-art strategies on each the regression and classification duties. The webserver and software program are freely obtainable at PEMPNI

Optimising glyphosate tolerance in rapeseed (Brassica napus L.) by CRISPR/Cas9-based geminiviral donor DNA replicon system with Csy4-based single-guide RNA processing

 

Rapeseed (Brassica napus L.) is a crucial oil crop worldwide, and efficient weed management can defend its yields and high quality. Farmers can profit from tolerant cultivars when utilizing herbicides, equivalent to glyphosate. Amino acid substitutions in enolpyruvylshikimate-3-phosphate synthase (EPSPS) render the plant much less delicate to glyphosate. Due to this fact, we aimed to optimise the glyphosate tolerance trait in rapeseed by way of endogenous EPSPS modification. To attain efficient gene substitute in B. napus L., we employed a CRISPR/Cas9 system expressing single-guide RNAs (sgRNAs) cleaved by the CRISPR-associated RNA endoribonuclease Csy4 from Pseudomonas aeruginosa for focused induction of double-strand breaks. Each the donor template and a geminiviral replicon harbouring an sgRNA expression cassette had been launched into plant cells.

Utilizing sgRNAs focusing on adjoining donor DNA template containing synonymous mutations in sgRNA websites, we achieved exact gene replacements within the endogenous B. napus EPSPS gene, BnaC04EPSPS, leading to TIPS and LFGAAGMCRL amino acid substitutions at frequencies as much as 20%. Rapeseed seedlings harbouring EPSPSmTIPS or EPSPSmLFGAAGMCRL had been glyphosate tolerant. Moreover, modifications in BnaC04EPSPS had been exactly transmitted to the following era. Our genome enhancing technique allows extremely environment friendly gene focusing on and the induction of glyphosate tolerance in oilseed rape.

DNA Oxidative Damage Kit - Quantitative
DN-002	Kamiya Biomedical Company	20 tests	EUR 656

OxiSelect™ Oxidative DNA Damage Quantitation Kit (AP sites)
STA-324	Cell Biolabs	50 assays	EUR 695

OxiSelect Oxidative DNA Damage Quantitation Kit (AP Sites), Trial Size
STA-324-T	Cell Biolabs	10 assays	EUR 498
Description: DNA damage can manifest in the formation of apurinic or apyrimidinic (AP or abasic) sites. Such spontaneous base loss in mammalian cells has been estimated at between 50,000 and 200,000 sites per day. Unrepaired abasic sites can inhibit transcription and may be mutagenic. Our OxiSelect Oxidative DNA Damage Quantitation Kit provides a simple, user-friendly method for the quantitaiton DNA damage in the form of abasic sites. The assay uses an Aldehyde Reactive Probe (ARP) to specifically react with an aldehyde group on the open ring of the AP site. This allows the AP site to be labeled with Biotin, followed by detection with Streptavidin-Enzyme conjugate.

OxiSelect Oxidative RNA Damage ELISA Kit (8-OHG Quantitation) (96 tests)
MBS168447-5x96Assays	MyBiosource	5x96Assays	EUR 4465

OxiSelect Oxidative RNA Damage ELISA Kit (8-OHG Quantitation) (96 tests)
MBS168447-96Assays	MyBiosource	96Assays	EUR 1125

OxiSelect Oxidative RNA Damage ELISA Kit (8-OHG Quantitation), Trial Size
STA-325-T	Cell Biolabs	32 assays	EUR 609.6
Description: Recently oxidative RNA damage has been described in conjunction with a wide variety of neurological diseases including Alzheimer?s disease, Parkinson?s disease, Dementia with Lewy Bodies, prion disease, and subacute sclerosing panencephalitis. We have developed an easy-to-use ELISA for the detection of the most common marker of RNA damage: 8-hydroxyguanosine (8-OHG).

OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (1 plate)
MBS168191-32Assays	MyBiosource	32Assays	EUR 615

OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (1 plate)
MBS168191-5x96Assays	MyBiosource	5x96Assays	EUR 4465

OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (1 plate)
MBS168191-96Assays	MyBiosource	96Assays	EUR 1125

OxiSelect™ Oxidative RNA Damage ELISA Kit (8-OHG Quantitation) (96 tests)
STA-325	Cell Biolabs	96 assays	EUR 885

OxiSelect™ Oxidative RNA Damage ELISA Kit (8-OHG Quantitation) (5x96 tests)
STA-325-5	Cell Biolabs	5 x 96 assays	EUR 3795

OxiSelect™ Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (1 plate)
STA-320	Cell Biolabs	96 assays	EUR 885

OxiSelect™ Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (5 plates)
STA-320-5	Cell Biolabs	5 x 96 assays	EUR 3795

OxiSelect™ Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation), Trial Size
STA-320-T	Cell Biolabs	32 assays	EUR 440

DNA/RNA Damage Antibody, 15A3; Anti-DNA/RNA Damage Antibody
MBS802475-0025mg	MyBiosource	0.025mg	EUR 265

DNA / RNA Damage Antibody
abx440040-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx440321-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx440602-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx440883-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx441164-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx441445-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx441726-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx442007-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx442288-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx442569-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx442850-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx443130-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx443410-100ug	Abbexa	100 ug	EUR 644.4

DNA / RNA Damage Antibody
abx443691-100ug	Abbexa	100 ug	EUR 678

DNA / RNA Damage Antibody
20-abx444884	Abbexa	Ask for price Ask for price	100 ug 25 ug

DNA / RNA Damage Antibody
abx443972-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx444253-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx444534-100ug	Abbexa	100 ug	EUR 661.2

DNA / RNA Damage Antibody
abx444884-100l	Abbexa	100 µl	EUR 337.5

DNA/RNA Damage Antibody
MBS8001907-0025mg	MyBiosource	0.025mg	EUR 265

DNA/RNA Damage Antibody
MBS8001907-5x0025mg	MyBiosource	5x0.025mg	EUR 925

DNA/RNA Damage Antibody
MBS801590-01mg	MyBiosource	0.1mg	EUR 415

DNA/RNA Damage Antibody
MBS801590-5x01mg	MyBiosource	5x0.1mg	EUR 1595

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
abx148136-100ug	Abbexa	100 ug	EUR 526.8

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
20-abx005629	Abbexa	Ask for price Ask for price Ask for price Ask for price	100 ul 200 ul 20 ul 50 ul

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
20-abx321453	Abbexa	Ask for price Ask for price	100 ul 50 ul

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
20-abx320325	Abbexa	Ask for price Ask for price	100 ul 50 ul

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
abx321453-100g	Abbexa	100 µg	EUR 350

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
abx321453-50g	Abbexa	50 µg	EUR 237.5

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
abx320325-100l	Abbexa	100 µl	EUR 350

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
abx320325-50l	Abbexa	50 µl	EUR 237.5

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody
abx005629-100l	Abbexa	100 µl	EUR 175

DNA / RNA Damage Antibody (PE)
abx444253-100g	Abbexa	100 µg	EUR 575

DNA / RNA Damage Antibody (APC)
abx442569-100l	Abbexa	100 µl	EUR 575

DNA / RNA Damage Antibody (HRP)
abx443410-100g	Abbexa	100 µg	EUR 562.5

DNA/RNA Damage Antibody: HRP
MBS801864-01mg	MyBiosource	0.1mg	EUR 450

DNA/RNA Damage Antibody: HRP
MBS801864-5x01mg	MyBiosource	5x0.1mg	EUR 1770

DNA/RNA Damage Antibody: RPE
MBS803622-01mg	MyBiosource	0.1mg	EUR 460

DNA/RNA Damage Antibody: RPE
MBS803622-5x01mg	MyBiosource	5x0.1mg	EUR 1820

DNA / RNA Damage Antibody (FITC)
abx443130-100g	Abbexa	100 µg	EUR 575

DNA/RNA Damage Antibody: FITC
MBS803261-01mg	MyBiosource	0.1mg	EUR 455

DNA/RNA Damage Antibody: FITC
MBS803261-5x01mg	MyBiosource	5x0.1mg	EUR 1790

DNA / RNA Damage Antibody (PerCP)
abx443972-100g	Abbexa	100 µg	EUR 575

DNA/RNA Damage Antibody: PerCP
MBS802697-01mg	MyBiosource	0.1mg	EUR 465

DNA/RNA Damage Antibody: PerCP
MBS802697-5x01mg	MyBiosource	5x0.1mg	EUR 1830

DNA / RNA Damage Antibody (Biotin)
abx442850-100g	Abbexa	100 µg	EUR 575

DNA/RNA Damage Antibody: Biotin
MBS803145-01mg	MyBiosource	0.1mg	EUR 460

DNA/RNA Damage Antibody: Biotin
MBS803145-5x01mg	MyBiosource	5x0.1mg	EUR 1815

DNA / RNA Damage Antibody (ATTO594)
abx440883-100g	Abbexa	100 µg	EUR 587.5

DNA / RNA Damage Antibody (ATTO390)
abx440040-200g	Abbexa	200 µg	EUR 587.5

DNA / RNA Damage Antibody (ATTO488)
abx440321-100g	Abbexa	100 µg	EUR 587.5

DNA/RNA Damage Antibody: ATTO 594
MBS800732-01mg	MyBiosource	0.1mg	EUR 465

DNA/RNA Damage Antibody: ATTO 594
MBS800732-5x01mg	MyBiosource	5x0.1mg	EUR 1835

DNA/RNA Damage Antibody: ATTO 390
MBS802627-01mg	MyBiosource	0.1mg	EUR 465

DNA/RNA Damage Antibody: ATTO 390
MBS802627-5x01mg	MyBiosource	5x0.1mg	EUR 1840

DNA/RNA Damage Antibody: ATTO 488
MBS804277-01mg	MyBiosource	0.1mg	EUR 465

DNA/RNA Damage Antibody: ATTO 488
MBS804277-5x01mg	MyBiosource	5x0.1mg	EUR 1835

DNA/RNA Damage Antibody, Clone 15A3
SMC-155C	Stressmarq	0.025mg	EUR 144
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

DNA/RNA Damage Antibody, Clone 15A3
SMC-155D	Stressmarq	0.1mg	EUR 306
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

DNA/RNA Damage Antibody, Clone 15A3
SMC-155S	Stressmarq	0.012mg	EUR 44
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

OASE00080-25UG - DNA/RNA Damage Antibody
OASE00080-25UG	Aviva Systems Biology	25ug	EUR 199

OASE00081-100UG - DNA/RNA Damage Antibody
OASE00081-100UG	Aviva Systems Biology	100ug	EUR 399

DNA/RNA Damage Antibody: Alkaline Phosphatase
MBS800170-01mg	MyBiosource	0.1mg	EUR 460

DNA/RNA Damage Antibody: Alkaline Phosphatase
MBS800170-5x01mg	MyBiosource	5x0.1mg	EUR 1800

DNA/RNA Damage Antibody, Clone 15A3: APC
SMC-155D-APC	Stressmarq	0.1mg	EUR 358
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC.

DNA/RNA Damage Antibody, Clone 15A3: HRP
SMC-155D-HRP	Stressmarq	0.1mg	EUR 345
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with HRP.

DNA/RNA Damage Antibody, Clone 15A3: RPE
SMC-155D-RPE	Stressmarq	0.1mg	EUR 355
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with RPE.

DNA/RNA Damage Antibody, Clone 15A3: FITC
SMC-155D-FITC	Stressmarq	0.1mg	EUR 350
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with FITC.

DNA/RNA Damage Antibody, Clone 15A3: PerCP
SMC-155D-PCP	Stressmarq	0.1mg	EUR 358
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with PerCP.

DNA/RNA Damage Antibody, Clone 15A3: Biotin
SMC-155D-BI	Stressmarq	0.1mg	EUR 354
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Biotin.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A565	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 565.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A633	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 633.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A655	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 655.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A680	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 680.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A700	Stressmarq	0.1mg	EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 700.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-APCCY7	Stressmarq	0.1mg	EUR 540
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC/Cy7.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY350	Stressmarq	0.1mg	EUR 471.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 350.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY405	Stressmarq	0.1mg	EUR 457.2
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 405.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY488	Stressmarq	0.1mg	EUR 445.2
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 488.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY594	Stressmarq	0.1mg	EUR 447.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 594.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY633	Stressmarq	0.1mg	EUR 441.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 633.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-P594	Stressmarq	0.1mg	EUR 462
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with PE/ATTO 594.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-STR	Stressmarq	0.1mg	EUR 451.2
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Streptavidin.

DNA/RNA Damage Antibody, Clone 15A3: ATTO 390
SMC-155D-A390	Stressmarq	0.1mg	EUR 360
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 390.

DNA/RNA Damage Antibody, Clone 15A3: ATTO 488
SMC-155D-A488	Stressmarq	0.1mg	EUR 359
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 488.

DNA/RNA Damage Antibody, Clone 15A3: ATTO 594
SMC-155D-A594	Stressmarq	0.1mg	EUR 359
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 594.

DNA/RNA Damage Antibody, Clone 15A3: Alkaline Phosphatase
SMC-155D-ALP	Stressmarq	0.1mg	EUR 352
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Alkaline Phosphatase.

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx114290-100l	Abbexa	100 µl	EUR 612.5

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx212012-100l	Abbexa	100 µl	EUR 350

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx212012-50l	Abbexa	50 µl	EUR 250

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx213481-10l	Abbexa	10 µl	EUR 250

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx236055-100g	Abbexa	100 µg	EUR 350

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx146245-1096tests	Abbexa	10 × 96 tests	Ask for price

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx146245-596tests	Abbexa	5 × 96 tests	Ask for price

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx146245-96tests	Abbexa	96 tests	EUR 337.5

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx325277-100g	Abbexa	100 µg	EUR 250

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx325277-50g	Abbexa	50 µg	EUR 187.5

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx325602-100g	Abbexa	100 µg	EUR 250

Oxidative Stress Responsive 1 (OXSR1) Antibody
abx325602-50g	Abbexa	50 µg	EUR 187.5

Human DNA Oxidative Demethylase ALKBH2 (ALKBH2) Protein
abx073206-20kDa1g	Abbexa	20 kDa; 1 g	EUR 225

Human DNA Oxidative Demethylase ALKBH2 (ALKBH2) Protein
abx073206-20kDa5g	Abbexa	20 kDa; 5 g	EUR 3550

Human DNA Oxidative Demethylase ALKBH2 (ALKBH2) Protein
abx073206-40kDa1g	Abbexa	40 kDa; 1 g	EUR 325

Oxidative-Stress Responsive 1 (OXSR1) Antibody
20-abx114290	Abbexa	Ask for price Ask for price	150 ul 50 ul

Recombinant Human DNA oxidative demethylase ALKBH2 (ALKBH2)
CSB-EP761219HU	Cusabio	4107 mg	Ask for price

Oxidative Stress Antibodies Set | 5 antibodies
AS19-4294	Agrisera AB	each	EUR 1472

A genome-wide display screen for differentially methylated lengthy noncoding RNAs recognized that lncAC007255.Eight is regulated by promoter DNA methylation in Beas-2B cells malignantly reworked by NNK

 Tobacco publicity is well-known to induce genetic and epigenetic adjustments that contribute to the pathogenesis of lung most cancers. 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a big tobacco-specific carcinogen, however the oncogenic mechanisms of NNK haven’t been completely elucidated. On this examine we discovered that DNA methyltransferase 1(DNMT1) was overexpressed in malignantly reworked human bronchial epithelial Beas-2B cells induced by NNK (2B-NNK cells), by therapy with NNK (400 μg/ml) for 7 days. An Arraystar Human noncoding RNA Promoter Microarray was used to detect the DNA methylation standing of the promoter area of lengthy noncoding RNAs (lncRNAs). The outcome confirmed that 1010 differentially methylated fragments had been current within the lncRNA promoter area. QRT-PCR revealed that the expression of lncRNA AC007255.Eight was remarkably downregulated in 2B-NNK cells and lung most cancers tissues.

Moreover, Methylation-particular PCR confirmed that the methylation of the lncRNA AC007255.Eight promoter was elevated in 2B-NNK cells and lung most cancers tissues. The lowered expression of lncRNA AC007255.Eight was considerably related to hypermethylation of lncRNA AC007255.Eight promoter area. LncRNA AC007255.Eight overexpression may lead to decreased cell proliferation and elevated cell apoptosis in 2B-NNK cells. In conclusion, NNK induced lncRNA AC007255.8 promoter hypermethylation by way of upregulation of DNMT1 in Beas-2B cells, resulting in downregulation of lncRNA AC007255.8, and finally the enhancement of cell proliferation and the inhibition of apoptosis. This analysis affords novel insights into the epigenetic mechanisms of lung most cancers, and can stimulate additional analysis into the involvement of aberrant DNA methylation of non-coding areas of the genome within the pathogenesis of lung most cancers.

Detecting protein and DNA/RNA constructions in cryo-EM maps of intermediate decision utilizing deep studying

 

An growing variety of density maps of macromolecular constructions, together with proteins and DNA/RNA complexes, have been decided by cryo-electron microscopy (cryo-EM). Though these days maps at a near-atomic decision are routinely reported, there are nonetheless substantial fractions of maps decided at intermediate or low resolutions, the place extracting construction data shouldn’t be trivial. Right here, we report a brand new computational methodology, Emap2sec+, which identifies DNA or RNA as properly because the secondary constructions of proteins in cryo-EM maps of 5 to 10 Å decision.

Emap2sec+ employs the deep Residual convolutional neural community. Emap2sec+ assigns structural labels with related possibilities at every voxel in a cryo-EM map, which can assist construction modeling in an EM map. Emap2sec+ confirmed secure and excessive task accuracy for nucleotides in low decision maps and improved efficiency for protein secondary construction assignments than its earlier model when examined on simulated and experimental maps.

 

Temporal panorama of human intestine RNA and DNA virome in SARS-CoV-2 an infection and severity

 

Background: Coronavirus illness 2019 (COVID-19) brought on by the enveloped RNA virus SARS-CoV-2 primarily impacts the respiratory and gastrointestinal tracts. SARS-CoV-2 was remoted from fecal samples, and energetic viral replication was reported in human intestinal cells. The human intestine additionally harbors an unlimited quantity of resident viruses (collectively referred to as the virome) that play a task in regulating host immunity and illness pathophysiology. Understanding intestine virome perturbation that underlies SARS-CoV-2 an infection and severity is an unmet want.

 

Strategies: We enrolled 98 COVID-19 sufferers with various illness severity (Three asymptomatic, 53 gentle, 34 reasonable, 5 extreme, Three important) and 78 non-COVID-19 controls matched for gender and co-morbidities. All topics had fecal specimens sampled at inclusion. Blood specimens had been collected for COVID-19 sufferers at admission to check for inflammatory markers and white cell counts. Amongst COVID-19 circumstances, 37 (38%) sufferers had serial fecal samples collected 2 to three occasions per week from time of hospitalization till after discharge. Utilizing shotgun metagenomics sequencing, we sequenced and profiled the fecal RNA and DNA virome. We investigated alterations and longitudinal dynamics of the intestine virome in affiliation with illness severity and blood parameters.

Outcomes: Sufferers with COVID-19 confirmed underrepresentation of Pepper gentle mottle virus (RNA virus) and a number of bacteriophage lineages (DNA viruses) and enrichment of environment-derived eukaryotic DNA viruses in fecal samples, in comparison with non-COVID-19 topics. Such intestine virome alterations continued as much as 30 days after illness decision. Fecal virome in SARS-CoV-2 an infection harbored extra stress-, inflammation-, and virulence-associated gene encoding capacities together with these pertaining to bacteriophage integration, DNA restore, and metabolism and virulence related to their bacterial host. Baseline fecal abundance of 10 virus species (1 RNA virus, pepper chlorotic spot virus, and 9 DNA virus species) inversely correlated with illness COVID-19 severity. These viruses inversely correlated with blood ranges of pro-inflammatory proteins, white cells, and neutrophils. Among the many 10 COVID-19 severity-associated DNA virus species, Four confirmed inverse correlation with age; 5 confirmed persistent decrease abundance each throughout illness course and after illness decision relative to non-COVID-19 topics.

Conclusions: Each enteric RNA and DNA virome in COVID-19 sufferers had been completely different from non-COVID-19 topics, which continued after illness decision of COVID-19. Intestine virome might calibrate host immunity and regulate severity to SARS-CoV-2 an infection. Our statement that intestine viruses inversely correlated with each severity of COVID-19 and host age might partly clarify that older topics are susceptible to extreme and worse COVID-19 outcomes. Altogether, our information spotlight the significance of human intestine virome in severity and probably therapeutics of COVID-19. Video Summary.