A genome-wide screen for differentially methylated long noncoding RNAs

Systematic comparability and prediction of the consequences of missense mutations on protein-DNA and protein-RNA interactions

 The binding affinities of protein-nucleic acid interactions may very well be altered as a consequence of missense mutations occurring in DNA- or RNA-binding proteins, subsequently leading to numerous illnesses. Sadly, a scientific comparability and prediction of the consequences of mutations on protein-DNA and protein-RNA interactions (these two mutation lessons are termed MPDs and MPRs, respectively) remains to be missing. Right here, we demonstrated that these two lessons of mutations may generate comparable or completely different tendencies for binding free power adjustments when it comes to the properties of mutated residues. We then developed regression algorithms individually for MPDs and MPRs by introducing novel geometric partition-based power options and interface-based structural options.

By characteristic choice and ensemble studying, comparable computational frameworks that built-in energy- and nonenergy-based fashions had been established to estimate the binding affinity adjustments ensuing from MPDs and MPRs, however the chosen options for the remaining fashions had been completely different and subsequently mirrored the specificity of those two mutation lessons. Moreover, the proposed methodology was prolonged to the identification of mutations that considerably decreased the binding affinities. In depth validations indicated that our algorithm typically carried out higher than the state-of-the-art strategies on each the regression and classification duties. The webserver and software program are freely obtainable at PEMPNI

Optimising glyphosate tolerance in rapeseed (Brassica napus L.) by CRISPR/Cas9-based geminiviral donor DNA replicon system with Csy4-based single-guide RNA processing

 

Rapeseed (Brassica napus L.) is a crucial oil crop worldwide, and efficient weed management can defend its yields and high quality. Farmers can profit from tolerant cultivars when utilizing herbicides, equivalent to glyphosate. Amino acid substitutions in enolpyruvylshikimate-3-phosphate synthase (EPSPS) render the plant much less delicate to glyphosate. Due to this fact, we aimed to optimise the glyphosate tolerance trait in rapeseed by way of endogenous EPSPS modification. To attain efficient gene substitute in B. napus L., we employed a CRISPR/Cas9 system expressing single-guide RNAs (sgRNAs) cleaved by the CRISPR-associated RNA endoribonuclease Csy4 from Pseudomonas aeruginosa for focused induction of double-strand breaks. Each the donor template and a geminiviral replicon harbouring an sgRNA expression cassette had been launched into plant cells.

Utilizing sgRNAs focusing on adjoining donor DNA template containing synonymous mutations in sgRNA websites, we achieved exact gene replacements within the endogenous B. napus EPSPS gene, BnaC04EPSPS, leading to TIPS and LFGAAGMCRL amino acid substitutions at frequencies as much as 20%. Rapeseed seedlings harbouring EPSPSmTIPS or EPSPSmLFGAAGMCRL had been glyphosate tolerant. Moreover, modifications in BnaC04EPSPS had been exactly transmitted to the following era. Our genome enhancing technique allows extremely environment friendly gene focusing on and the induction of glyphosate tolerance in oilseed rape.

bio-tech-inc

bio-tech-inc

OxiSelect™ Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (5 plates)

STA-320-5 5 x 96 assays
EUR 3795

OxiSelect™ Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation), Trial Size

STA-320-T 32 assays
EUR 440

DNA / RNA Damage Antibody

abx440040-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx440321-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx440602-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx440883-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx441164-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx441445-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx441726-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx442007-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx442288-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx442569-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx442850-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx443130-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx443410-100ug 100 ug
EUR 644.4

DNA / RNA Damage Antibody

abx443691-100ug 100 ug
EUR 678

DNA / RNA Damage Antibody

abx443972-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx444253-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

abx444534-100ug 100 ug
EUR 661.2

DNA / RNA Damage Antibody

20-abx444884
  • Ask for price
  • Ask for price
  • 100 ug
  • 25 ug

DNA / RNA Damage Antibody

abx444884-100l 100 µl
EUR 337.5

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody

20-abx321453
  • Ask for price
  • Ask for price
  • 100 ul
  • 50 ul

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody

20-abx320325
  • Ask for price
  • Ask for price
  • 100 ul
  • 50 ul

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody

abx148136-100ug 100 ug
EUR 526.8

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody

20-abx005629
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody

abx005629-100l 100 µl
EUR 175

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody

abx320325-100l 100 µl
EUR 350

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody

abx320325-50l 50 µl
EUR 237.5

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody

abx321453-100g 100 µg
EUR 350

DNA Oxidative Demethylase ALKBH2 (ALKBH2) Antibody

abx321453-50g 50 µg
EUR 237.5

DNA / RNA Damage Antibody (PE)

abx444253-100g 100 µg
EUR 575

DNA / RNA Damage Antibody (APC)

abx442569-100l 100 µl
EUR 575

DNA / RNA Damage Antibody (HRP)

abx443410-100g 100 µg
EUR 562.5

DNA / RNA Damage Antibody (FITC)

abx443130-100g 100 µg
EUR 575

DNA / RNA Damage Antibody (PerCP)

abx443972-100g 100 µg
EUR 575

DNA / RNA Damage Antibody (Biotin)

abx442850-100g 100 µg
EUR 575

DNA / RNA Damage Antibody (ATTO488)

abx440321-100g 100 µg
EUR 587.5

DNA / RNA Damage Antibody (ATTO594)

abx440883-100g 100 µg
EUR 587.5

DNA / RNA Damage Antibody (ATTO390)

abx440040-200g 200 µg
EUR 587.5

DNA/RNA Damage Antibody, Clone 15A3

SMC-155C 0.025mg
EUR 144
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

DNA/RNA Damage Antibody, Clone 15A3

SMC-155D 0.1mg
EUR 306
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

DNA/RNA Damage Antibody, Clone 15A3

SMC-155S 0.012mg
EUR 44
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

OASE00080-25UG - DNA/RNA Damage Antibody

OASE00080-25UG 25ug
EUR 199

OASE00081-100UG - DNA/RNA Damage Antibody

OASE00081-100UG 100ug
EUR 399

DNA/RNA Damage Antibody, Clone 15A3: APC

SMC-155D-APC 0.1mg
EUR 358
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC.

DNA/RNA Damage Antibody, Clone 15A3: HRP

SMC-155D-HRP 0.1mg
EUR 345
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with HRP.

DNA/RNA Damage Antibody, Clone 15A3: RPE

SMC-155D-RPE 0.1mg
EUR 355
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with RPE.

DNA/RNA Damage Antibody, Clone 15A3: FITC

SMC-155D-FITC 0.1mg
EUR 350
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with FITC.

DNA/RNA Damage Antibody, Clone 15A3: PerCP

SMC-155D-PCP 0.1mg
EUR 358
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with PerCP.

DNA/RNA Damage Antibody, Clone 15A3: Biotin

SMC-155D-BI 0.1mg
EUR 354
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Biotin.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-A565 0.1mg
EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 565.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-A633 0.1mg
EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 633.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-A655 0.1mg
EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 655.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-A680 0.1mg
EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 680.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-A700 0.1mg
EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 700.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-APCCY7 0.1mg
EUR 540
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC/Cy7.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-DY350 0.1mg
EUR 471.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 350.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-DY405 0.1mg
EUR 457.2
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 405.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-DY488 0.1mg
EUR 445.2
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 488.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-DY594 0.1mg
EUR 447.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 594.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-DY633 0.1mg
EUR 441.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 633.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-P594 0.1mg
EUR 462
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with PE/ATTO 594.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-STR 0.1mg
EUR 451.2
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Streptavidin.

DNA/RNA Damage Antibody, Clone 15A3: ATTO 390

SMC-155D-A390 0.1mg
EUR 360
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 390.

DNA/RNA Damage Antibody, Clone 15A3: ATTO 488

SMC-155D-A488 0.1mg
EUR 359
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 488.

DNA/RNA Damage Antibody, Clone 15A3: ATTO 594

SMC-155D-A594 0.1mg
EUR 359
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 594.

DNA/RNA Damage Antibody, Clone 15A3: Alkaline Phosphatase

SMC-155D-ALP 0.1mg
EUR 352
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Alkaline Phosphatase.

Human DNA Oxidative Demethylase ALKBH2 (ALKBH2) Protein

abx073206-20kDa1g 20 kDa; 1 g
EUR 225

Human DNA Oxidative Demethylase ALKBH2 (ALKBH2) Protein

abx073206-20kDa5g 20 kDa; 5 g
EUR 3550

Human DNA Oxidative Demethylase ALKBH2 (ALKBH2) Protein

abx073206-40kDa1g 40 kDa; 1 g
EUR 325

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx236055-100g 100 µg
EUR 350

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx114290-100l 100 µl
EUR 612.5

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx146245-1096tests 10 × 96 tests Ask for price

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx146245-596tests 5 × 96 tests Ask for price

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx146245-96tests 96 tests
EUR 337.5

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx212012-100l 100 µl
EUR 350

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx212012-50l 50 µl
EUR 250

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx213481-10l 10 µl
EUR 250

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx325277-100g 100 µg
EUR 250

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx325277-50g 50 µg
EUR 187.5

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx325602-100g 100 µg
EUR 250

Oxidative Stress Responsive 1 (OXSR1) Antibody

abx325602-50g 50 µg
EUR 187.5

Recombinant Human DNA oxidative demethylase ALKBH2 (ALKBH2)

CSB-EP761219HU 4107 mg Ask for price

Oxidative-Stress Responsive 1 (OXSR1) Antibody

20-abx114290
  • Ask for price
  • Ask for price
  • 150 ul
  • 50 ul

Anti-8-Oxo-A DNA/RNA Damage Mouse Monoclonal Antibody

12503 100ug
EUR 230

Anti-8-Oxo-A DNA/RNA Damage Mouse Monoclonal Antibody

12503-100 100ug
EUR 230

Anti-8-Oxo-A DNA/RNA Damage Mouse Monoclonal Antibody

12503-500 500ug
EUR 460

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

20-abx173928
  • Ask for price
  • Ask for price
  • 1 mg
  • 200 ug

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

20-abx128290
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx236027-100ug 100 ug
EUR 610.8

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx146030-100ug 100 ug
EUR 469.2

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

20-abx114289
  • Ask for price
  • Ask for price
  • 150 ul
  • 50 ul

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx029975-400ul 400 ul
EUR 627.6

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx029975-80l 80 µl
EUR 343.2

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx236027-100g 100 µg
EUR 350

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx128290-100l 100 µl
EUR 275

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx128290-1ml 1 ml
EUR 750

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx128290-200l 200 µl
EUR 337.5

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx114289-100l 100 µl
EUR 612.5

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx029975-400l 400 µl
EUR 518.75

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx146030-1096tests 10 × 96 tests Ask for price

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx146030-596tests 5 × 96 tests Ask for price

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx146030-96tests 96 tests
EUR 337.5

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx173928-100l 100 µl
EUR 787.5

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx173928-1ml 1 ml Ask for price

Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Antibody

abx173928-200l 200 µl Ask for price

Human Oxidative Stress-Responsive 1 Protein (OXSR1) Antibody

33219-05111 150 ug
EUR 215

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody

20-abx302885
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody

abx122927-100ug 100 ug
EUR 469.2

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody

abx302885-100g 100 µg
EUR 362.5

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody

abx302885-20g 20 µg
EUR 162.5

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody

abx302885-50g 50 µg
EUR 250

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody

abx122927-100l 100 µl Ask for price

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody

abx122927-20l 20 µl
EUR 337.5

Oxidative Stress-Induced Growth Inhibitor 2 (OSGIN2) Antibody

abx122927-50l 50 µl Ask for price

Thiol Oxidative Stress Assay

1021 1 kit
EUR 460.8

Recombinant Human DNA oxidative demethylase ALKBH2 Protein, GST, E.coli-10ug

QP7771-ec-10ug 10ug
EUR 240

Recombinant Human DNA oxidative demethylase ALKBH2 Protein, GST, E.coli-1mg

QP7771-ec-1mg 1mg
EUR 1958.4

Recombinant Human DNA oxidative demethylase ALKBH2 Protein, GST, E.coli-50ug

QP7771-ec-50ug 50ug
EUR 315.6

Recombinant Human DNA oxidative demethylase ALKBH2 Protein, GST, E.coli-100ug

QP7771-ec-100ug 100ug
EUR 489.6

A genome-wide display screen for differentially methylated lengthy noncoding RNAs recognized that lncAC007255.Eight is regulated by promoter DNA methylation in Beas-2B cells malignantly reworked by NNK

 Tobacco publicity is well-known to induce genetic and epigenetic adjustments that contribute to the pathogenesis of lung most cancers. 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a big tobacco-specific carcinogen, however the oncogenic mechanisms of NNK haven’t been completely elucidated. On this examine we discovered that DNA methyltransferase 1(DNMT1) was overexpressed in malignantly reworked human bronchial epithelial Beas-2B cells induced by NNK (2B-NNK cells), by therapy with NNK (400 μg/ml) for 7 days. An Arraystar Human noncoding RNA Promoter Microarray was used to detect the DNA methylation standing of the promoter area of lengthy noncoding RNAs (lncRNAs). The outcome confirmed that 1010 differentially methylated fragments had been current within the lncRNA promoter area. QRT-PCR revealed that the expression of lncRNA AC007255.Eight was remarkably downregulated in 2B-NNK cells and lung most cancers tissues.

Moreover, Methylation-particular PCR confirmed that the methylation of the lncRNA AC007255.Eight promoter was elevated in 2B-NNK cells and lung most cancers tissues. The lowered expression of lncRNA AC007255.Eight was considerably related to hypermethylation of lncRNA AC007255.Eight promoter area. LncRNA AC007255.Eight overexpression may lead to decreased cell proliferation and elevated cell apoptosis in 2B-NNK cells. In conclusion, NNK induced lncRNA AC007255.8 promoter hypermethylation by way of upregulation of DNMT1 in Beas-2B cells, resulting in downregulation of lncRNA AC007255.8, and finally the enhancement of cell proliferation and the inhibition of apoptosis. This analysis affords novel insights into the epigenetic mechanisms of lung most cancers, and can stimulate additional analysis into the involvement of aberrant DNA methylation of non-coding areas of the genome within the pathogenesis of lung most cancers.

Detecting protein and DNA/RNA constructions in cryo-EM maps of intermediate decision utilizing deep studying

 

An growing variety of density maps of macromolecular constructions, together with proteins and DNA/RNA complexes, have been decided by cryo-electron microscopy (cryo-EM). Though these days maps at a near-atomic decision are routinely reported, there are nonetheless substantial fractions of maps decided at intermediate or low resolutions, the place extracting construction data shouldn’t be trivial. Right here, we report a brand new computational methodology, Emap2sec+, which identifies DNA or RNA as properly because the secondary constructions of proteins in cryo-EM maps of 5 to 10 Å decision.

Emap2sec+ employs the deep Residual convolutional neural community. Emap2sec+ assigns structural labels with related possibilities at every voxel in a cryo-EM map, which can assist construction modeling in an EM map. Emap2sec+ confirmed secure and excessive task accuracy for nucleotides in low decision maps and improved efficiency for protein secondary construction assignments than its earlier model when examined on simulated and experimental maps.

 

Temporal panorama of human intestine RNA and DNA virome in SARS-CoV-2 an infection and severity

 

Background: Coronavirus illness 2019 (COVID-19) brought on by the enveloped RNA virus SARS-CoV-2 primarily impacts the respiratory and gastrointestinal tracts. SARS-CoV-2 was remoted from fecal samples, and energetic viral replication was reported in human intestinal cells. The human intestine additionally harbors an unlimited quantity of resident viruses (collectively referred to as the virome) that play a task in regulating host immunity and illness pathophysiology. Understanding intestine virome perturbation that underlies SARS-CoV-2 an infection and severity is an unmet want.

 

Strategies: We enrolled 98 COVID-19 sufferers with various illness severity (Three asymptomatic, 53 gentle, 34 reasonable, 5 extreme, Three important) and 78 non-COVID-19 controls matched for gender and co-morbidities. All topics had fecal specimens sampled at inclusion. Blood specimens had been collected for COVID-19 sufferers at admission to check for inflammatory markers and white cell counts. Amongst COVID-19 circumstances, 37 (38%) sufferers had serial fecal samples collected 2 to three occasions per week from time of hospitalization till after discharge. Utilizing shotgun metagenomics sequencing, we sequenced and profiled the fecal RNA and DNA virome. We investigated alterations and longitudinal dynamics of the intestine virome in affiliation with illness severity and blood parameters.

Outcomes: Sufferers with COVID-19 confirmed underrepresentation of Pepper gentle mottle virus (RNA virus) and a number of bacteriophage lineages (DNA viruses) and enrichment of environment-derived eukaryotic DNA viruses in fecal samples, in comparison with non-COVID-19 topics. Such intestine virome alterations continued as much as 30 days after illness decision. Fecal virome in SARS-CoV-2 an infection harbored extra stress-, inflammation-, and virulence-associated gene encoding capacities together with these pertaining to bacteriophage integration, DNA restore, and metabolism and virulence related to their bacterial host. Baseline fecal abundance of 10 virus species (1 RNA virus, pepper chlorotic spot virus, and 9 DNA virus species) inversely correlated with illness COVID-19 severity. These viruses inversely correlated with blood ranges of pro-inflammatory proteins, white cells, and neutrophils. Among the many 10 COVID-19 severity-associated DNA virus species, Four confirmed inverse correlation with age; 5 confirmed persistent decrease abundance each throughout illness course and after illness decision relative to non-COVID-19 topics.

Conclusions: Each enteric RNA and DNA virome in COVID-19 sufferers had been completely different from non-COVID-19 topics, which continued after illness decision of COVID-19. Intestine virome might calibrate host immunity and regulate severity to SARS-CoV-2 an infection. Our statement that intestine viruses inversely correlated with each severity of COVID-19 and host age might partly clarify that older topics are susceptible to extreme and worse COVID-19 outcomes. Altogether, our information spotlight the significance of human intestine virome in severity and probably therapeutics of COVID-19. Video Summary.